منابع مشابه
Oocyte-sperm interactions.
The penetration of the zona pellucida is a crucial step during fertilization. Spermatozoa that are unable to recognize and bind to the zona pellucida glycoproteins or respond to the zona pellucida by undergoing the acrosome reaction fail to fertilize the egg. In most mammalian species, after entering the fallopian tube sperm are stored in the isthmic part of the oviduct under conditions that ma...
متن کاملIntracytoplasmic sperm injection: laboratory set-up and injection procedure.
Intracytoplasmic sperm injection (ICSI) as treatment for male-factor infertility has been introduced worldwide in the past few years in many laboratories using assisted reproduction techniques. Some changes in the existing set-up are necessary before implementing this procedure. The equipment can be divided into two groups: that required for preparation of the microtools and that required for t...
متن کاملP-19: Effect of Three Sperm Preparation Methods on Sperm DNA Integrity and Oocyte Fertilization Rate
Background: Techniques of sperm preparation require centrifugation to separate spermatozoa from the seminal plasma. The centrifugation can induce damage to the spermatozoa and increase reactive oxygen species(ROS) productionspecially in leukospermic speciemens that are associated with impaired function and fertilizing capacity. However in ICSI, fertilization can occure with damage DNA sperm but...
متن کاملSperm-oocyte interaction in the sea-urchin.
A study was made of the electrical and morphological changes in the sea-urchin oocyte during interaction with spermatozoa. The first event, a small step depolarization accompanied by a 20-40% decay in input resistance occurs within seconds of attachment. The evidence suggests that this electrical event is the result of sperm-oocyte fusion, and that the ion channels that lower the resistance acr...
متن کاملSperm and oocyte isolation methods for biochemical and proteomic analysis.
Caenorhabditis elegans gonad is a simple model to investigate molecular mechanisms that regulate fundamental cell and developmental processes. The strength of the model is that C. elegans is amenable to genetic manipulation. The complete genome sequence, advances in mass spectrometry, and RNA-mediated interference (RNAi) are now providing a technical infrastructure that complements biochemical ...
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ژورنال
عنوان ژورنال: Current Trends in Clinical Embriology
سال: 2017
ISSN: 2385-2836
DOI: 10.11138/cce/2017.4.2.049